Title:	Comprehensive examination of mesophases formed by DMPC and DHPC mixtures
Authors:	Harroun, T.A. Koslowsky, Martin Nieh, M.P. de Lannoy, C.F. Raghunathan, V.A. Katsaras, J.
Issue Date:	12-May-2005
Publisher:	American Chemical Society
Citation:	Langmuir, 2005, Vol. 21, p5356-5361
Abstract:	Mixtures of long- and short-chain phospholipids, specifically 14:0 and 6:0 phosphatidylcholines (DMPC and DHPC), have been used successfully in NMR studies as magnetically alignable substrates for membrane-associated proteins. However, recent publications have shown that the phase behavior of these mixtures is much more complex than originally thought. Using polarized light microscopy and small-angle neutron scattering, phase diagrams of DMPC/DHPC mixtures at molar ratios of 2, 3.2, and 5 have been determined. Generally, at temperatures below the main-chain melting transition of DMPC (TM = 23 C), an isotropic phase of disklike micelles is found. At high temperatures (T > 50 C), a lamellar phase consisting of either multilamellar vesicles (MLV) or extended lamellae is formed, which at low lipid concentrations (e.g., MLV) coexists with an excess of water. At intermediate temperatures and lipid concentrations, a chiral nematic phase made up of wormlike micelles was observed.
Description:	Restricted Access.
URI:	http://hdl.handle.net/2289/2263
ISSN:	0743-7463
Alternative Location:	http://dx.doi.org/10.1021/la050018t
Copyright:	2005 American Chemical Society
Appears in Collections:	Research Papers (SCM)

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