Title:	Quantitative analysis of intra-Golgi transport shows intercisternal exchange for all cargo
Authors:	Dmitrieff, Serge Rao, Madan Sens, Pierre
Keywords:	Golgi apparatus quantitative transport model resident golgi enzymes convection-diffusion
Issue Date:	24-Sep-2013
Publisher:	National Academy of Sciences of the United States of America
Citation:	Proceedings of the National Academy of Sciences of the United States of America, 2013, Vol.110, p15692-15697
Abstract:	The mechanisms controlling the transport of proteins through the Golgi stack of mammalian and plant cells is the subject of intense debate, with two models, cisternal progression and intercisternal exchange, emerging as major contenders. A variety of transport experiments have claimed support for each of these models. We reevaluate these experiments using a single quantitative coarse-grained framework of intra-Golgi transport that accounts for both transport models and their many variants. Our analysis makes a definitive case for the existence of intercisternal exchange both for small membrane proteins and large protein complexes––this implies that membrane structures larger than the typical protein-coated vesicles must be involved in transport. Notwithstanding, we find that current observations on protein transport cannot rule out cisternal progression as contributing significantly to the transport process. To discriminate between the different models of intra-Golgi transport, we suggest experiments and an analysis based on our extended theoretical framework that compare the dynamics of transiting and resident proteins.
Description:	Open Access
URI:	http://hdl.handle.net/2289/5950
ISSN:	1091-6490 (online)
Alternative Location:	http://arxiv.org/abs/1307.7043 http://dx.doi.org/10.1073/pnas.1303358110 http://adsabs.harvard.edu/abs/2013PNAS..11015692D
Copyright:	2013 National Academy of Sciences of the United States of America This article may be downloaded for personal use only. Any other use requires prior permission of the author and the National Academy of Sciences of the United States of America
Appears in Collections:	Research Papers (TP)

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